Fluorescent bioinspired albumin/polydopamine nanoparticles and their interactions with Escherichia coli cells

• Eloïse Equy,
• Jordana Hirtzel,
• Sophie Hellé,
• Béatrice Heurtault,
• Eric Mathieu,
• Morgane Rabineau,
• Vincent Ball and
• Lydie Ploux

Beilstein J. Nanotechnol. 2023, 14, 1208–1224, doi:10.3762/bjnano.14.100

• ) of poly(lactic-co-glycolic acid) (PLGA) [7], polycaprolactone [8], and chitosan [9]. Furthermore, fluorescent ONPs are a promising way to facilitate the localization of NPs in cells through fluorescence imaging. They can also be used for fluorescent labelling of cells, especially for live cell

• high-resolution fluorescence imaging (high-resolution confocal microscopy). Also, the capacity of the pristine and fluorescent NPs to inhibit the growth of bacterial cells was evaluated through minimal inhibitory concentration (MIC) tests. The MIC value was also determined for Staphylococcus aureus (S

Recent progress in cancer cell membrane-based nanoparticles for biomedical applications

• Qixiong Lin,
• Yueyou Peng,
• Yanyan Wen,
• Xiaoqiong Li,
• Donglian Du,
• Weibin Dai,
• Wei Tian and
• Yanfeng Meng

Beilstein J. Nanotechnol. 2023, 14, 262–279, doi:10.3762/bjnano.14.24

• -encapsulated PLGA nanospheres loaded with doxorubicin (Dox-HepM-PLGA) yielded smaller tumor volumes than the bare nanoparticles and the PBS control group. (a) Fluorescence imaging eleven days after intravenous injection of biomimetic nanoformulations. (b) Tumor volume. (c) Tumor weight. (d) Relative tumor

• fluorescent dye Cy5.5 (Cy5.5@HMnO2-AM) in mice. (A) In vivo fluorescence imaging. (B) Statistical analysis of the fluorescence intensity of tumors in different treatment groups. (C) Mouse image. (D) Ex vivo fluorescence imaging of major organs. (E) T1-weighted MR image of the biomimetic nanoparticles. (F) T1

Structural, optical, and bioimaging characterization of carbon quantum dots solvothermally synthesized from o-phenylenediamine

• Zoran M. Marković,
• Milica D. Budimir,
• Martin Danko,
• Dušan D. Milivojević,
• Pavel Kubat,
• Danica Z. Zmejkoski,
• Vladimir B. Pavlović,
• Marija M. Mojsin,
• Milena J. Stevanović and
• Biljana M. Todorović Marković

Beilstein J. Nanotechnol. 2023, 14, 165–174, doi:10.3762/bjnano.14.17

• test internalization of CQDs, Hela cells were treated for 48 h with a concentration of 200 µg/mL CQDs. As shown in Figure 6b, fluorescence imaging demonstrated that CQDs penetrated Hela cells well (compared to Hela cells treated with vehicle control, shown in Figure 6a) and were mainly in the cytoplasm

Biomimetic chitosan with biocomposite nanomaterials for bone tissue repair and regeneration

• Se-Kwon Kim,
• Sesha Subramanian Murugan,
• Pandurang Appana Dalavi,
• Sebanti Gupta,
• Sukumaran Anil,
• Gi Hun Seong and
• Jayachandran Venkatesan

Beilstein J. Nanotechnol. 2022, 13, 1051–1067, doi:10.3762/bjnano.13.92

• were identified by fluorescence imaging. Cells adhered on nanofibers show a better cell phenotype, and this was corroborated by morphological characterisation via SEM [72] (Figure 6). Misra and colleagues developed chitosan–graphene nanocomposite scaffolds that modify cell–scaffold interactions

Detection and imaging of Hg(II) in vivo using glutathione-functionalized gold nanoparticles

• Gufeng Li,
• Shaoqing Li,
• Rui Wang,
• Min Yang,
• Lizhu Zhang,
• Yanli Zhang,
• Wenrong Yang and
• Hongbin Wang

Beilstein J. Nanotechnol. 2022, 13, 549–559, doi:10.3762/bjnano.13.46

• GNPs-GSH-Rh6G2 containing different amounts of Hg2+. Real-time fluorescence imaging of HeLa cells treated with (a–c) GSH-Rh6G2 and (d–f) GNPs-GSH-Rh6G2 together with 100 µL with Hg2+ after different incubation times (0, 1.5 and 2.5 h). The scale bars are 100 µm. From left to right, the images represent

• fluorescence, bright-field, and merged-channel fluorescence imaging. (g) Evaluation of cytotoxicity on HeLa cells of GSH-Rh6G2 and GNPs-GSH-Rh6G2 at different concentrations (0.01, 0.02, 0.03, 0.04, and 0.05 µmol) after incubation for 24 h. Fluorescence intensity of RGCOOH released from GNPs-GSH-Rh6G2 in the

Theranostic potential of self-luminescent branched polyethyleneimine-coated superparamagnetic iron oxide nanoparticles

• Rouhollah Khodadust,
• Ozlem Unal and
• Havva Yagci Acar

Beilstein J. Nanotechnol. 2022, 13, 82–95, doi:10.3762/bjnano.13.6

• in many in vitro studies including, for example, flow cytometry or fluorescence imaging, since the luminescence of the polymer was not detected [18][33][34]. Unfortunately, the luminescence of the fluorophores (dye or quantum dots) that are active in the visible range is usually significantly reduced

• mean. The statistical analysis was conducted using ANOVA and two-sample unequal variances were used to calculate the p-values between groups. All cell viability percentages were presented as percentages of the control viability. Fluorescence imaging To investigate the in vitro optical imaging potential

Comprehensive review on ultrasound-responsive theranostic nanomaterials: mechanisms, structures and medical applications

• Sepand Tehrani Fateh,
• Lida Moradi,
• Elmira Kohan,
• Michael R. Hamblin and
• Amin Shiralizadeh Dezfuli

Beilstein J. Nanotechnol. 2021, 12, 808–862, doi:10.3762/bjnano.12.64

Fusion of purple membranes triggered by immobilization on carbon nanomembranes

• René Riedel,
• Natalie Frese,
• Fang Yang,
• Martin Wortmann,
• Raphael Dalpke,
• Daniel Rhinow,
• Norbert Hampp and
• Armin Gölzhäuser

Beilstein J. Nanotechnol. 2021, 12, 93–101, doi:10.3762/bjnano.12.8

• the final linker is shown in Figure 2a. The functionalization was tested with the His-tagged fluorescent protein mTurqoise. Confirmation of successful functionalization was provided by fluorescence imaging as seen in Figure 2b. The images show that the complex bonds formed with His-labeled proteins

Thermophoretic tweezers for single nanoparticle manipulation

• Jošt Stergar and
• Natan Osterman

Beilstein J. Nanotechnol. 2020, 11, 1126–1133, doi:10.3762/bjnano.11.97

• implemented using a custom-built fluorescence microscope. By using an LED-optimized filter cube (Dapi/FITC/Cy3/Cy5 Quad LED HC Filter Set, AHF analysentechnik AG), Köhler illumination is utilized for fluorescence imaging using an LED as its light source. Light is collected from the sample plane by a 63

Applications of superparamagnetic iron oxide nanoparticles in drug and therapeutic delivery, and biotechnological advancements

• Maria Suciu,
• Corina M. Ionescu,
• Alexandra Ciorita,
• Septimiu C. Tripon,
• Dragos Nica,
• Hani Al-Salami and
• Lucian Barbu-Tudoran

Beilstein J. Nanotechnol. 2020, 11, 1092–1109, doi:10.3762/bjnano.11.94

• been extensively examined regarding biocompatibility, targeted or intended cytotoxicity (ferroptosis), local hyperthermia treatments, photothermal or photodynamic therapy, and MRI. Also, there are increasingly more studies reporting on combinations with in vivo fluorescence imaging, sensing and

• for MRI and fluorescence imaging with good cytocompatibility. Park et al. [161] synthesized SPIONs coated with folate containing 64Cu for positronic emission tomography and MRI. Cai et al. [162] obtained 12 nm SPIONs coated with a near-infrared fluorescent dye for dual in vivo imagistics (MRI and

• graphitic-phase carbon nitride and coated with polyethylene glycol for MRI and fluorescence imaging and for photodynamic therapy. This type of nanoplatform seems to be a good one-for-all solution, as it could be controlled to be non-toxic or highly toxic from the outside. In vitro and in vivo analyses

Key for crossing the BBB with nanoparticles: the rational design

• Sonia M. Lombardo,
• Marc Schneider,
• Akif E. Türeli and
• Nazende Günday Türeli

Beilstein J. Nanotechnol. 2020, 11, 866–883, doi:10.3762/bjnano.11.72

• fluorescence imaging [68]. Nanoparticles could be observed in the glioma bed and infiltrating margin, showing that nanoparticles functionalized with angiopep-2 could exhibit dual-targeting abilities. Firstly, angiopep-2 allowed the nanoparticles to cross the BBB through RMT by recognition of LRP1 on the BBB

Examination of the relationship between viscoelastic properties and the invasion of ovarian cancer cells by atomic force microscopy

• Mengdan Chen,
• Jinshu Zeng,
• Weiwei Ruan,
• Zhenghong Zhang,
• Yuhua Wang,
• Shusen Xie,
• Zhengchao Wang and
• Hongqin Yang

Beilstein J. Nanotechnol. 2020, 11, 568–582, doi:10.3762/bjnano.11.45

• understanding the molecular mechanism regulating the relationship between the viscoelastic and tumorigenic properties in ovarian cancer cells, the microfilament density of F-actin cytoskeleton was examined by fluorescence imaging of these cells after treatment with 0.25 μM Ech for 0, 3 and 6 h (Figure 7). The

• stained with ActinGreen (KeyGEN BioTECH). A laser scanning confocal microscope (SP8, Leica) was used to image the cytoskeletal organization by F-actin. The fluorescence imaging was captured at 488 nm excitation wavelength. Moreover, the images were processed with the software Image J. Statistical analysis

Luminescent gold nanoclusters for bioimaging applications

• Nonappa

Beilstein J. Nanotechnol. 2020, 11, 533–546, doi:10.3762/bjnano.11.42

• [Au25(ZWMe2)18] NCs. Intravenous injection of [Au25(ZWMe2)18] and in vivo fluorescence imaging after 1 h showed a strong signal in the bladder indicating a high and fast renal clearance. Further, a strong fluorescence in the NIR region (that of NCs) was observed in urine generated during the first hour

• in the liver. The tumor uptake studies were performed for [Au25(SG)18] and [Au25(ZWMe2)18] intravenously injected in mice bearing a subcutaneous U87MG tumor by tracking the NCs using fluorescence imaging. No signal was detected after 5 or 24 h for [Au25(SG)18]. However, strong fluorescence was

• permission from [53], copyright 2019 Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim. In vivo bioimaging using luminescent AuNCs. A) NIR fluorescence imaging of BALB/c mouse treated with the Au-BSA NCs. The blue line shows the autofluorescence and Au-BSA NCs signal in red in the fluorescence spectra. B) In vivo

Rational design of block copolymer self-assemblies in photodynamic therapy

• Maxime Demazeau,
• Laure Gibot,
• Anne-Françoise Mingotaud,
• Patricia Vicendo,
• Clément Roux and
• Barbara Lonetti

Beilstein J. Nanotechnol. 2020, 11, 180–212, doi:10.3762/bjnano.11.15

• is an example of “all in one“ nanomedicine used for chemotherapy combining loading with doxorubicin, PDT and PTT. This is possible thanks to the activation of the photosensitizer and multimodal imaging using the fluorescence of the photosensitizer (near-infrared fluorescence imaging, NIRFI) and the

Evaluation of click chemistry microarrays for immunosensing of alpha-fetoprotein (AFP)

• Seyed Mohammad Mahdi Dadfar,
• Sylwia Sekula-Neuner,
• Vanessa Trouillet,
• Hui-Yu Liu,
• Ravi Kumar,
• Annie K. Powell and
• Michael Hirtz

Beilstein J. Nanotechnol. 2019, 10, 2505–2515, doi:10.3762/bjnano.10.241

• system onboard software (NanoScope 8.10, Bruker, Germany). The XPS analysis was performed using a K-Alpha+ XPS spectrometer (ThermoFisher Scientific, East Grinstead, UK) using the Thermo Avantage software as previously described [47]. Sample analysis was performed as reported in [25]. Fluorescence

• imaging The fluorescently labeled surface patterns were imaged using a Nikon Eclipse 80i upright fluorescence microscope (Nikon, Japan) equipped with an Intensilight illumination (Nikon, Japan), a CoolSNAP HQ2 camera (Photometrics, USA) and a Texas Red set (Y-2E/C, Nikon). Statistical analysis The data

Nitrogen-vacancy centers in diamond for nanoscale magnetic resonance imaging applications

• Alberto Boretti,
• Lorenzo Rosa,
• Jonathan Blackledge and
• Stefania Castelletto

Beilstein J. Nanotechnol. 2019, 10, 2128–2151, doi:10.3762/bjnano.10.207

Engineered superparamagnetic iron oxide nanoparticles (SPIONs) for dual-modality imaging of intracranial glioblastoma via EGFRvIII targeting

• Xianping Liu,
• Chengjuan Du,
• Haichun Li,
• Ting Jiang,
• Zimiao Luo,
• Zhiqing Pang,
• Daoying Geng and
• Jun Zhang

Beilstein J. Nanotechnol. 2019, 10, 1860–1872, doi:10.3762/bjnano.10.181

• seeded into 24-well plates and cultured for 24 h. When the cells reached 80% confluence, they were incubated with 5-FAM-labled PEPHC1 (1 mg/mL) for 1 h. For fluorescence imaging, the culture solution was discarded, and the cells were incubated with 4% paraformaldehyde solution for 15 minutes at room

• , multiple echo times (TE) 8 ms, 16 ms, 24 ms, 32 ms, 40 ms, 48 ms, 56 ms, 64 ms, repetition time (TR) 1500 ms. The quantitative assay of the signal intensity was measured at the center of the tumor area with an operator-defined region of interest (ROI). In vivo fluorescence imaging Two weeks after U87MG

• of 20 mg of Fe/kg and fluorescence imaging was conducted at various time points (2 h, 4 h, 8 h, and 24 h) after intravenous injection using an IVIS spectrum imaging system (PerkinElmer, USA). The excitation wavelength was 788 nm and the emission wavelength of 808 nm of Cy7.5 was selected. 24 hours

Polydopamine-coated Au nanorods for targeted fluorescent cell imaging and photothermal therapy

• Boris N. Khlebtsov,
• Andrey M. Burov,
• Timofey E. Pylaev and
• Nikolai G. Khlebtsov

Beilstein J. Nanotechnol. 2019, 10, 794–803, doi:10.3762/bjnano.10.79

• concentrations of AuNR-PDA-R123-folate or AuNR-PDA-R123-PEG after (A) 24 h and (B) 48 h of incubation. (C) The microscopy images of HeLa (F+) and HEK 293 (F−) cells after treatment with AuNR-PDA-R123-folate or AuNR-PDA-R123-PEG for 2 h. BF: bright-field imaging; Fluor: fluorescence imaging. Temperature changes

Enhanced antineoplastic/therapeutic efficacy using 5-fluorouracil-loaded calcium phosphate nanoparticles

• Shanid Mohiyuddin,
• Saba Naqvi and
• Gopinath Packirisamy

Beilstein J. Nanotechnol. 2018, 9, 2499–2515, doi:10.3762/bjnano.9.233

• of 2 μL of 10 mg/mL Hoechst 33342 (incubated for 5 min) then observed under an EVOS fluorescence imaging system with a DAPI filter for the Hoechst 33342 blue fluorescence. This was followed by a PBS wash and addition of 3 µL of rhodamine B (1 mg/mL, incubated for 5–10 min) at 37 °C. Finally, images

• of the cells were captured by using blue (DAPI) and red (RFP) filters in the EVOS fluorescence imaging system and superimposed during image capturing. Membrane blebbing determination by FE-SEM Once the understanding of apoptotic induction was confirmed by AO/EB staining, the membrane blebbing

Correction: Photobleaching of YOYO-1 in super-resolution single DNA fluorescence imaging

• Joseph R. Pyle and
• Jixin Chen

Beilstein J. Nanotechnol. 2018, 9, 809–811, doi:10.3762/bjnano.9.74

Green synthesis of fluorescent carbon dots from spices for in vitro imaging and tumour cell growth inhibition

• Nagamalai Vasimalai,
• Vânia Vilas-Boas,
• Juan Gallo,
• María de Fátima Cerqueira,
• Mario Menéndez-Miranda,
• José Manuel Costa-Fernández,
• Lorena Diéguez,
• Begoña Espiña and
• María Teresa Fernández-Argüelles

Beilstein J. Nanotechnol. 2018, 9, 530–544, doi:10.3762/bjnano.9.51

• results obtained in cell viability studies, fluorescence imaging experiments were conducted after the incubation of LN-229 and HK-2 cells for 24 h, with each C-dot solution (spice- and citrate-based C-dots) at a concentration of 1 mg·mL−1. Confocal fluorescence imaging shows a diffuse accumulation of the

Nanoparticle delivery to metastatic breast cancer cells by nanoengineered mesenchymal stem cells

• Liga Saulite,
• Karlis Pleiko,
• Ineta Popena,
• Dominyka Dapkute,
• Ricardas Rotomskis and
• Una Riekstina

Beilstein J. Nanotechnol. 2018, 9, 321–332, doi:10.3762/bjnano.9.32

• plot. Importantly, 96% of the QD-loaded MSC population was CD90 positive in 3D monoculture (Figure 6C). As expected, MCF7 (Figure 6D) and MDA-MB-231 cells (Figure 6E) were CD90 negative in 3D monocultures. The QD transfer from MSCs to cancer cells was also visualised by fluorescence imaging where CD90

• dissociation of the spheroids into a single cell suspension. The cells were washed, stained with CD90 FITC or EpCAM FITC and analysed by flow cytometry. For fluorescence imaging of QD transfer, the cancer cell and MSC mono- or co-culture spheroids were harvested after 24 h of propagation on the polyHEMA

• . The samples were then counterstained with Hoechst 33342 trihydrochloride (10 mg/mL) solution (Thermo Fisher Scientific, USA), fixed with 4% PFA and mounted with ProLong gold anti-fade mounting medium. Fluorescence imaging For the z-scan measurements, MSC spheroids were stained with 5 μM 3,3

Patterning of supported gold monolayers via chemical lift-off lithography

• Liane S. Slaughter,
• Kevin M. Cheung,
• Sami Kaappa,
• Huan H. Cao,
• Qing Yang,
• Thomas D. Young,
• Andrew C. Serino,
• Sami Malola,
• Jana M. Olson,
• Stephan Link,
• Hannu Häkkinen,
• Anne M. Andrews and
• Paul S. Weiss

Beilstein J. Nanotechnol. 2017, 8, 2648–2661, doi:10.3762/bjnano.8.265

• DNA, or self-assembly and hybridization of DNA on unpatterned PDMS (Figure S5, Supporting Information File 1). Using CLL and fluorescence imaging, we produced images over square-millimeter areas with a lateral feature size spanning several orders of magnitude on the same substrates (Figure 2C,D). We

Photobleaching of YOYO-1 in super-resolution single DNA fluorescence imaging

• Joseph R. Pyle and
• Jixin Chen

Beilstein J. Nanotechnol. 2017, 8, 2296–2306, doi:10.3762/bjnano.8.229

• theoretically predicted with the proposed method in this report. Keywords: diffusion; PAINT; single-molecule photophysics; super-resolution imaging; Introduction Fluorescence imaging of DNA with intercalating dyes is important for DNA sensing [1][2], nucleic acid imaging inside cells and viruses [3][4][5

• , height). The height is defined by the thickness of the tape. Fluorescence imaging All fluorescence measurements were carried out with a home-built microscope under total internal reflection fluorescence (TIRF) mode (Figure 2) equipped with four solid state lasers (Dragon Lasers, China), two beam

Carbon nano-onions as fluorescent on/off modulated nanoprobes for diagnostics

• Stefania Lettieri,
• Marta d’Amora,
• Adalberto Camisasca,
• Alberto Diaspro and
• Silvia Giordani

Beilstein J. Nanotechnol. 2017, 8, 1878–1888, doi:10.3762/bjnano.8.188

• fluorescent CNOs is fast and reversible both in solution and in vitro, making this nanomaterial suitable as pH-dependent probes for diagnostic applications. Keywords: carbon nanomaterials; fluorescence; imaging; nanomedicine; nano-onion; Introduction Nanomaterial-based probes (nano-probes) that are able to

• %) and PBS and incubated with a solution of Hoechst 33342 (5 μg mL−1) (Sigma) for 20 min. Finally, the cells were rinsed three times and filled with PBS. Confocal fluorescence imaging was then carried out with a Nikon A1R laser scanning microscope and a plan apo 20× DIC M and a plan apo VC 60× oil DIC N2